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| 19.09.2009 | 16:00 | Viktoria Plzeň | - | FK Mladá Boleslav | 2:1 (1:1) | |
| 18:15 | FK Baumit Jablonec | - | Slovan Liberec | 2:1 (1:1) | ||
| 20.09.2009 | 17:00 | Bohemians Praha | - | 1. FK Příbram | 1:0 (1:0) | |
| 17:00 | Dynamo České Budějovice | - | 1. FC Slovácko | 0:0 (0:0) | ||
| 17:00 | FK Teplice | - | Bohemians 1905 | 1:0 (0:0) | ||
| 17:00 | Sigma Olomouc | - | SK Kladno | 1:2 (0:1) | ||
| 21.09.2009 | 17:30 | Slavia Praha | - | Baník Ostrava | 3:1 (1:1) | |
| 19:30 | 1. FC Brno | - | AC Sparta Praha | 1:1 (0:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Teplice | 8 | 5 | 3 | 0 | 15:6 | 9 | 18 |
| 2 |
|
FK Baumit Jablonec | 8 | 5 | 2 | 1 | 11:8 | 3 | 17 |
| 3 |
|
Slavia Praha | 8 | 4 | 3 | 1 | 13:7 | 6 | 15 |
| 4 |
|
AC Sparta Praha | 8 | 3 | 5 | 0 | 8:3 | 5 | 14 |
| 5 |
|
Baník Ostrava | 8 | 4 | 2 | 2 | 9:8 | 1 | 14 |
| 6 |
|
Viktoria Plzeň | 8 | 4 | 1 | 3 | 11:10 | 1 | 13 |
| 7 |
|
1. FC Brno | 8 | 3 | 3 | 2 | 8:6 | 2 | 12 |
| 8 |
|
FK Mladá Boleslav | 8 | 3 | 2 | 3 | 13:9 | 4 | 11 |
| 9 |
|
1. FK Příbram | 8 | 3 | 2 | 3 | 7:7 | 0 | 11 |
| 10 |
|
Bohemians 1905 | 8 | 2 | 3 | 3 | 4:4 | 0 | 9 |
| 11 |
|
Slovan Liberec | 8 | 2 | 2 | 4 | 11:13 | -2 | 8 |
| 12 |
|
Sigma Olomouc | 8 | 1 | 4 | 3 | 8:10 | -2 | 7 |
| 13 |
|
1. FC Slovácko | 8 | 2 | 1 | 5 | 8:12 | -4 | 7 |
| 14 |
|
SK Kladno | 8 | 2 | 1 | 5 | 6:12 | -6 | 7 |
| 15 |
|
Dynamo České Budějovice | 8 | 1 | 2 | 5 | 4:12 | -8 | 5 |
| 16 |
|
Bohemians Praha | 8 | 2 | 0 | 6 | 5:14 | -9 | -14 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
