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| 31.10.2009 | 16:00 | 1. FK Příbram | - | Slavia Praha | 1:0 (0:0) | |
| 18:15 | AC Sparta Praha | - | Baník Ostrava | 1:1 (0:0) | ||
| 01.11.2009 | 15:00 | FK Baumit Jablonec | - | Bohemians Praha | 1:0 (0:0) | |
| 15:00 | SK Kladno | - | FK Teplice | 0:2 (0:0) | ||
| 17:00 | Bohemians 1905 | - | Slovan Liberec | 0:1 (0:0) | ||
| 17:00 | FK Mladá Boleslav | - | 1. FC Slovácko | 3:2 (3:0) | ||
| 17:00 | Sigma Olomouc | - | Dynamo České Budějovice | 2:0 (1:0) | ||
| 02.11.2009 | 17:30 | Viktoria Plzeň | - | 1. FC Brno | 1:1 (0:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Teplice | 13 | 8 | 5 | 0 | 26:10 | 16 | 29 |
| 2 |
|
FK Baumit Jablonec | 13 | 9 | 2 | 2 | 15:9 | 6 | 29 |
| 3 |
|
Baník Ostrava | 13 | 7 | 4 | 2 | 18:12 | 6 | 25 |
| 4 |
|
AC Sparta Praha | 13 | 5 | 8 | 0 | 14:7 | 7 | 23 |
| 5 |
|
Viktoria Plzeň | 13 | 6 | 4 | 3 | 14:11 | 3 | 22 |
| 6 |
|
FK Mladá Boleslav | 13 | 5 | 4 | 4 | 22:16 | 6 | 19 |
| 7 |
|
Slavia Praha | 13 | 5 | 4 | 4 | 17:12 | 5 | 19 |
| 8 |
|
Slovan Liberec | 13 | 5 | 2 | 6 | 15:16 | -1 | 17 |
| 9 |
|
1. FK Příbram | 13 | 5 | 2 | 6 | 12:14 | -2 | 17 |
| 10 |
|
1. FC Brno | 13 | 4 | 5 | 4 | 11:14 | -3 | 17 |
| 11 |
|
Sigma Olomouc | 13 | 4 | 4 | 5 | 17:13 | 4 | 16 |
| 12 |
|
1. FC Slovácko | 13 | 4 | 1 | 8 | 13:19 | -6 | 13 |
| 13 |
|
Bohemians 1905 | 13 | 2 | 5 | 6 | 6:10 | -4 | 11 |
| 14 |
|
SK Kladno | 13 | 3 | 2 | 8 | 9:20 | -11 | 11 |
| 15 |
|
Dynamo České Budějovice | 13 | 1 | 5 | 7 | 6:18 | -12 | 8 |
| 16 |
|
Bohemians Praha | 13 | 2 | 1 | 10 | 11:25 | -14 | -13 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
