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| 14.05.2021 | 20:00 | Slovan Liberec | - | Zbrojovka Brno | 1:1 (0:0) | |
| 15.05.2021 | 15:00 | SFC Opava | - | Sigma Olomouc | 1:2 (0:1) | |
| 17:00 | Baník Ostrava | - | FC Fastav Zlín | 4:2 (2:0) | ||
| 17:00 | 1. FK Příbram | - | FK Jablonec | 1:0 (0:0) | ||
| 19:30 | 1. FC Slovácko | - | Bohemians Praha 1905 | 1:1 (1:1) | ||
| 16.05.2021 | 15:00 | FK Pardubice | - | Viktoria Plzeň | 3:0 (1:0) | |
| 15:00 | FK Teplice | - | Dynamo České Budějovice | 2:0 (0:0) | ||
| 17:00 | FK Mladá Boleslav | - | AC Sparta Praha | 4:5 (2:2) | ||
| 19:30 | Slavia Praha | - | MFK Karviná | 1:1 (1:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 32 | 25 | 7 | 0 | 82:18 | 64 | 82 |
| 2 |
|
AC Sparta Praha | 32 | 21 | 5 | 6 | 74:41 | 33 | 68 |
| 3 |
|
FK Jablonec | 32 | 20 | 5 | 7 | 57:32 | 25 | 65 |
| 4 |
|
1. FC Slovácko | 32 | 17 | 6 | 9 | 55:33 | 22 | 57 |
| 5 |
|
Viktoria Plzeň | 32 | 15 | 7 | 10 | 53:44 | 9 | 52 |
| 6 |
|
Slovan Liberec | 32 | 14 | 9 | 9 | 43:30 | 13 | 51 |
| 7 |
|
FK Pardubice | 32 | 14 | 7 | 11 | 40:41 | -1 | 49 |
| 8 |
|
Baník Ostrava | 32 | 13 | 9 | 10 | 47:33 | 14 | 48 |
| 9 |
|
Bohemians Praha 1905 | 32 | 10 | 13 | 9 | 38:32 | 6 | 43 |
| 10 |
|
Sigma Olomouc | 32 | 10 | 12 | 10 | 38:36 | 2 | 42 |
| 11 |
|
Dynamo České Budějovice | 32 | 9 | 11 | 12 | 32:44 | -12 | 38 |
| 12 |
|
MFK Karviná | 32 | 8 | 11 | 13 | 34:48 | -14 | 35 |
| 13 |
|
FK Mladá Boleslav | 32 | 8 | 9 | 15 | 43:53 | -10 | 33 |
| 14 |
|
FC Fastav Zlín | 32 | 8 | 7 | 17 | 30:48 | -18 | 31 |
| 15 |
|
FK Teplice | 32 | 7 | 8 | 17 | 32:62 | -30 | 29 |
| 16 |
|
1. FK Příbram | 32 | 5 | 9 | 18 | 24:62 | -38 | 24 |
| 17 |
|
Zbrojovka Brno | 32 | 4 | 11 | 17 | 28:50 | -22 | 23 |
| 18 |
|
SFC Opava | 32 | 3 | 8 | 21 | 21:64 | -43 | 17 |
| Champions League Quali. | |
| Europa League Quali. | |
| Relégation |
