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| 26.02.2021 | 18:00 | Slovan Liberec | - | FK Pardubice | 4:1 (4:0) | |
| 27.02.2021 | 14:00 | SFC Opava | - | FK Jablonec | 0:1 (0:0) | |
| 16:00 | Baník Ostrava | - | FK Teplice | 1:1 (0:0) | ||
| 16:00 | Bohemians Praha 1905 | - | Dynamo České Budějovice | 1:1 (0:0) | ||
| 18:30 | Zbrojovka Brno | - | Viktoria Plzeň | 0:1 (0:0) | ||
| 28.02.2021 | 14:00 | FK Mladá Boleslav | - | MFK Karviná | 2:0 (2:0) | |
| 14:00 | Sigma Olomouc | - | FC Fastav Zlín | 0:1 (0:1) | ||
| 16:00 | 1. FC Slovácko | - | Slavia Praha | 2:3 (2:1) | ||
| 09.03.2021 | 18:00 | AC Sparta Praha | - | 1. FK Příbram | 4:0 (2:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 21 | 17 | 4 | 0 | 61:14 | 47 | 55 |
| 2 |
|
AC Sparta Praha | 21 | 15 | 2 | 4 | 47:24 | 23 | 47 |
| 3 |
|
1. FC Slovácko | 21 | 12 | 4 | 5 | 41:21 | 20 | 40 |
| 4 |
|
FK Jablonec | 21 | 12 | 3 | 6 | 36:25 | 11 | 39 |
| 5 |
|
Slovan Liberec | 21 | 10 | 6 | 5 | 32:19 | 13 | 36 |
| 6 |
|
Viktoria Plzeň | 21 | 10 | 4 | 7 | 38:26 | 12 | 34 |
| 7 |
|
FK Pardubice | 21 | 9 | 4 | 8 | 21:28 | -7 | 31 |
| 8 |
|
Sigma Olomouc | 21 | 7 | 9 | 5 | 29:24 | 5 | 30 |
| 9 |
|
Baník Ostrava | 21 | 8 | 6 | 7 | 26:21 | 5 | 30 |
| 10 |
|
Dynamo České Budějovice | 21 | 7 | 9 | 5 | 25:25 | 0 | 30 |
| 11 |
|
FC Fastav Zlín | 21 | 7 | 4 | 10 | 21:29 | -8 | 25 |
| 12 |
|
MFK Karviná | 21 | 6 | 7 | 8 | 24:33 | -9 | 25 |
| 13 |
|
Bohemians Praha 1905 | 21 | 6 | 6 | 9 | 23:27 | -4 | 24 |
| 14 |
|
FK Teplice | 21 | 5 | 4 | 12 | 20:42 | -22 | 19 |
| 15 |
|
FK Mladá Boleslav | 21 | 3 | 7 | 11 | 24:36 | -12 | 16 |
| 16 |
|
Zbrojovka Brno | 21 | 3 | 5 | 13 | 16:35 | -19 | 14 |
| 17 |
|
SFC Opava | 21 | 2 | 6 | 13 | 13:40 | -27 | 12 |
| 18 |
|
1. FK Příbram | 21 | 2 | 6 | 13 | 16:44 | -28 | 12 |
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