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| 05.03.2010 | 20:00 | AGOVV | - | MVV | 5:1 (5:0) | |
| 20:00 | FC Eindhoven | - | FC Emmen | 1:2 (1:0) | ||
| 20:00 | FC Volendam | - | RBC Roosendaal | 3:3 (1:2) | ||
| 20:00 | SBV Excelsior | - | FC Oss | 4:4 (3:3) | ||
| 20:00 | SC Cambuur | - | FC Zwolle | 3:0 (0:0) | ||
| 20:00 | FC Den Bosch | - | Helmond Sport | 2:1 (1:0) | ||
| 20:00 | Go Ahead Eagles | - | FC Dordrecht | 1:2 (0:1) | ||
| 20:00 | Fortuna Sittard | - | Telstar | 2:0 (1:0) | ||
| 30.03.2010 | 20:00 | BV Veendam | - | FC Omniworld | 0:1 (0:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 28 | 18 | 6 | 4 | 56:23 | 33 | 60 |
| 2 |
|
SC Cambuur | 28 | 16 | 8 | 4 | 66:41 | 25 | 56 |
| 3 |
|
Go Ahead Eagles | 28 | 15 | 8 | 5 | 45:22 | 23 | 53 |
| 4 |
|
FC Zwolle | 29 | 15 | 6 | 8 | 44:32 | 12 | 51 |
| 5 |
|
AGOVV | 29 | 14 | 8 | 7 | 54:41 | 13 | 48 |
| 6 |
|
SBV Excelsior | 28 | 14 | 5 | 9 | 55:40 | 15 | 47 |
| 7 |
|
Helmond Sport | 28 | 13 | 5 | 10 | 56:45 | 11 | 44 |
| 8 |
|
FC Den Bosch | 29 | 11 | 10 | 8 | 53:42 | 11 | 43 |
| 9 |
|
BV Veendam | 29 | 11 | 8 | 10 | 46:39 | 7 | 40 |
| 10 |
|
RBC Roosendaal | 29 | 10 | 8 | 11 | 37:43 | -6 | 38 |
| 11 |
|
FC Dordrecht | 28 | 11 | 3 | 14 | 48:47 | 1 | 36 |
| 12 |
|
FC Eindhoven | 28 | 10 | 5 | 13 | 50:61 | -11 | 35 |
| 13 |
|
MVV | 29 | 10 | 6 | 13 | 46:57 | -11 | 35 |
| 14 |
|
FC Omniworld | 28 | 9 | 5 | 14 | 31:48 | -17 | 32 |
| 15 |
|
FC Volendam | 28 | 6 | 11 | 11 | 49:59 | -10 | 29 |
| 16 |
|
FC Emmen | 29 | 8 | 5 | 16 | 39:65 | -26 | 29 |
| 17 |
|
Fortuna Sittard | 29 | 6 | 7 | 16 | 26:47 | -21 | 25 |
| 18 |
|
Telstar | 28 | 6 | 5 | 17 | 35:52 | -17 | 23 |
| 19 |
|
FC Oss | 28 | 3 | 9 | 16 | 35:67 | -32 | 18 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
