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| 05.02.2010 | 20:00 | SC Cambuur | - | AGOVV | 1:1 (1:0) | |
| 20:00 | Go Ahead Eagles | - | FC Volendam | 0:0 (0:0) | ||
| 20:00 | Telstar | - | FC Omniworld | 1:2 (1:2) | ||
| 20:00 | MVV | - | FC Dordrecht | 1:0 (1:0) | ||
| 20:00 | FC Den Bosch | - | BV Veendam | 2:2 (2:1) | ||
| 20:00 | Helmond Sport | - | FC Zwolle | 2:0 (0:0) | ||
| 20:00 | Fortuna Sittard | - | SBV Excelsior | 2:1 (2:0) | ||
| 15.02.2010 | 20:00 | De Graafschap | - | RBC Roosendaal | 0:0 (0:0) | |
| 01.03.2010 | 20:00 | FC Emmen | - | FC Oss | 3:3 (3:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 25 | 16 | 6 | 3 | 48:19 | 29 | 54 |
| 2 |
|
Go Ahead Eagles | 25 | 14 | 7 | 4 | 42:19 | 23 | 49 |
| 3 |
|
SC Cambuur | 24 | 13 | 8 | 3 | 51:35 | 16 | 47 |
| 4 |
|
FC Zwolle | 25 | 13 | 5 | 7 | 41:28 | 13 | 44 |
| 5 |
|
SBV Excelsior | 24 | 13 | 4 | 7 | 45:29 | 16 | 43 |
| 6 |
|
FC Den Bosch | 25 | 9 | 9 | 7 | 47:36 | 11 | 36 |
| 7 |
|
BV Veendam | 25 | 10 | 7 | 8 | 42:34 | 8 | 36 |
| 8 |
|
AGOVV | 25 | 10 | 8 | 7 | 42:37 | 5 | 36 |
| 9 |
|
Helmond Sport | 24 | 10 | 5 | 9 | 45:41 | 4 | 35 |
| 10 |
|
FC Dordrecht | 25 | 10 | 3 | 12 | 44:41 | 3 | 33 |
| 11 |
|
RBC Roosendaal | 25 | 9 | 6 | 10 | 30:34 | -4 | 33 |
| 12 |
|
MVV | 25 | 10 | 3 | 12 | 42:49 | -7 | 32 |
| 13 |
|
FC Eindhoven | 24 | 9 | 5 | 10 | 47:55 | -8 | 32 |
| 14 |
|
FC Volendam | 24 | 6 | 9 | 9 | 42:48 | -6 | 27 |
| 15 |
|
FC Omniworld | 25 | 7 | 4 | 14 | 26:46 | -20 | 25 |
| 16 |
|
Telstar | 24 | 6 | 5 | 13 | 33:45 | -12 | 23 |
| 17 |
|
Fortuna Sittard | 25 | 5 | 6 | 14 | 22:42 | -20 | 21 |
| 18 |
|
FC Emmen | 25 | 5 | 5 | 15 | 30:58 | -28 | 20 |
| 19 |
|
FC Oss | 24 | 3 | 7 | 14 | 29:52 | -23 | 16 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
