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| 01.05.2021 | 15:00 | 1. FK Příbram | - | Dynamo České Budějovice | 0:1 (0:1) | |
| 15:00 | SFC Opava | - | Bohemians Praha 1905 | 1:1 (1:0) | ||
| 17:00 | 1. FC Slovácko | - | FK Mladá Boleslav | 0:1 (0:1) | ||
| 19:30 | Baník Ostrava | - | Zbrojovka Brno | 1:1 (0:0) | ||
| 02.05.2021 | 15:00 | FK Jablonec | - | Sigma Olomouc | 3:1 (1:1) | |
| 15:00 | FK Pardubice | - | MFK Karviná | 2:2 (1:1) | ||
| 15:00 | FK Teplice | - | FC Fastav Zlín | 0:0 (0:0) | ||
| 17:00 | Slovan Liberec | - | AC Sparta Praha | 2:2 (1:0) | ||
| 19:30 | Slavia Praha | - | Viktoria Plzeň | 5:1 (2:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 30 | 24 | 6 | 0 | 80:17 | 63 | 78 |
| 2 |
|
AC Sparta Praha | 30 | 19 | 5 | 6 | 66:36 | 30 | 62 |
| 3 |
|
FK Jablonec | 30 | 19 | 5 | 6 | 53:30 | 23 | 62 |
| 4 |
|
1. FC Slovácko | 30 | 16 | 5 | 9 | 52:32 | 20 | 53 |
| 5 |
|
Viktoria Plzeň | 30 | 15 | 6 | 9 | 50:38 | 12 | 51 |
| 6 |
|
Slovan Liberec | 30 | 14 | 8 | 8 | 42:26 | 16 | 50 |
| 7 |
|
FK Pardubice | 30 | 13 | 7 | 10 | 37:39 | -2 | 46 |
| 8 |
|
Baník Ostrava | 30 | 12 | 9 | 9 | 42:28 | 14 | 45 |
| 9 |
|
Bohemians Praha 1905 | 30 | 9 | 12 | 9 | 34:31 | 3 | 39 |
| 10 |
|
Sigma Olomouc | 30 | 9 | 12 | 9 | 36:34 | 2 | 39 |
| 11 |
|
Dynamo České Budějovice | 30 | 8 | 11 | 11 | 30:42 | -12 | 35 |
| 12 |
|
MFK Karviná | 30 | 8 | 10 | 12 | 33:45 | -12 | 34 |
| 13 |
|
FK Mladá Boleslav | 30 | 7 | 9 | 14 | 36:46 | -10 | 30 |
| 14 |
|
FC Fastav Zlín | 30 | 8 | 6 | 16 | 27:43 | -16 | 30 |
| 15 |
|
FK Teplice | 30 | 6 | 8 | 16 | 29:58 | -29 | 26 |
| 16 |
|
Zbrojovka Brno | 30 | 4 | 10 | 16 | 25:46 | -21 | 22 |
| 17 |
|
1. FK Příbram | 30 | 4 | 8 | 18 | 20:59 | -39 | 20 |
| 18 |
|
SFC Opava | 30 | 3 | 7 | 20 | 19:61 | -42 | 16 |
| Champions League Quali. | |
| Europa League Quali. | |
| Relégation |
