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| 24.04.2021 | 14:00 | Dynamo České Budějovice | - | FK Jablonec | 0:2 (0:2) | |
| 14:00 | Sigma Olomouc | - | Baník Ostrava | 0:2 (0:1) | ||
| 16:00 | FK Mladá Boleslav | - | Slovan Liberec | 0:1 (0:1) | ||
| 18:30 | Viktoria Plzeň | - | 1. FC Slovácko | 2:1 (1:1) | ||
| 25.04.2021 | 14:00 | Zbrojovka Brno | - | FK Teplice | 0:0 (0:0) | |
| 14:00 | FC Fastav Zlín | - | FK Pardubice | 0:4 (0:2) | ||
| 14:00 | MFK Karviná | - | 1. FK Příbram | 0:1 (0:1) | ||
| 16:00 | AC Sparta Praha | - | SFC Opava | 4:2 (2:0) | ||
| 18:30 | Bohemians Praha 1905 | - | Slavia Praha | 0:0 (0:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 29 | 23 | 6 | 0 | 75:16 | 59 | 75 |
| 2 |
|
AC Sparta Praha | 29 | 19 | 4 | 6 | 64:34 | 30 | 61 |
| 3 |
|
FK Jablonec | 29 | 18 | 5 | 6 | 50:29 | 21 | 59 |
| 4 |
|
1. FC Slovácko | 29 | 16 | 5 | 8 | 52:31 | 21 | 53 |
| 5 |
|
Viktoria Plzeň | 29 | 15 | 6 | 8 | 49:33 | 16 | 51 |
| 6 |
|
Slovan Liberec | 29 | 14 | 7 | 8 | 40:24 | 16 | 49 |
| 7 |
|
FK Pardubice | 29 | 13 | 6 | 10 | 35:37 | -2 | 45 |
| 8 |
|
Baník Ostrava | 29 | 12 | 8 | 9 | 41:27 | 14 | 44 |
| 9 |
|
Sigma Olomouc | 29 | 9 | 12 | 8 | 35:31 | 4 | 39 |
| 10 |
|
Bohemians Praha 1905 | 29 | 9 | 11 | 9 | 33:30 | 3 | 38 |
| 11 |
|
MFK Karviná | 29 | 8 | 9 | 12 | 31:43 | -12 | 33 |
| 12 |
|
Dynamo České Budějovice | 29 | 7 | 11 | 11 | 29:42 | -13 | 32 |
| 13 |
|
FC Fastav Zlín | 29 | 8 | 5 | 16 | 27:43 | -16 | 29 |
| 14 |
|
FK Mladá Boleslav | 29 | 6 | 9 | 14 | 35:46 | -11 | 27 |
| 15 |
|
FK Teplice | 29 | 6 | 7 | 16 | 29:58 | -29 | 25 |
| 16 |
|
Zbrojovka Brno | 29 | 4 | 9 | 16 | 24:45 | -21 | 21 |
| 17 |
|
1. FK Příbram | 29 | 4 | 8 | 17 | 20:58 | -38 | 20 |
| 18 |
|
SFC Opava | 29 | 3 | 6 | 20 | 18:60 | -42 | 15 |
| Champions League Quali. | |
| Europa League Quali. | |
| Relégation |
