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| 19.03.2021 | 18:00 | FK Pardubice | - | FK Mladá Boleslav | 2:2 (1:0) | |
| 20.03.2021 | 14:00 | 1. FK Příbram | - | Zbrojovka Brno | 1:1 (0:1) | |
| 16:00 | MFK Karviná | - | Sigma Olomouc | 0:1 (0:0) | ||
| 18:30 | FK Jablonec | - | Slovan Liberec | 2:1 (1:0) | ||
| 21.03.2021 | 14:00 | 1. FC Slovácko | - | Baník Ostrava | 2:1 (2:0) | |
| 14:00 | FK Teplice | - | Bohemians Praha 1905 | 1:1 (0:0) | ||
| 16:00 | Dynamo České Budějovice | - | AC Sparta Praha | 0:0 (0:0) | ||
| 18:30 | Slavia Praha | - | SFC Opava | 4:0 (1:0) | ||
| 14.04.2021 | 16:00 | Viktoria Plzeň | - | FC Fastav Zlín | 2:0 (1:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 24 | 20 | 4 | 0 | 70:15 | 55 | 64 |
| 2 |
|
AC Sparta Praha | 24 | 16 | 3 | 5 | 50:26 | 24 | 51 |
| 3 |
|
FK Jablonec | 24 | 15 | 3 | 6 | 41:27 | 14 | 48 |
| 4 |
|
1. FC Slovácko | 24 | 13 | 5 | 6 | 44:25 | 19 | 44 |
| 5 |
|
Slovan Liberec | 24 | 12 | 6 | 6 | 37:22 | 15 | 42 |
| 6 |
|
Viktoria Plzeň | 24 | 12 | 4 | 8 | 42:29 | 13 | 40 |
| 7 |
|
FK Pardubice | 24 | 10 | 5 | 9 | 26:32 | -6 | 35 |
| 8 |
|
Sigma Olomouc | 24 | 8 | 10 | 6 | 30:25 | 5 | 34 |
| 9 |
|
Baník Ostrava | 24 | 9 | 6 | 9 | 33:25 | 8 | 33 |
| 10 |
|
Dynamo České Budějovice | 24 | 7 | 10 | 7 | 26:31 | -5 | 31 |
| 11 |
|
Bohemians Praha 1905 | 24 | 7 | 8 | 9 | 26:28 | -2 | 29 |
| 12 |
|
FC Fastav Zlín | 24 | 8 | 5 | 11 | 24:31 | -7 | 29 |
| 13 |
|
MFK Karviná | 24 | 6 | 8 | 10 | 25:37 | -12 | 26 |
| 14 |
|
FK Teplice | 24 | 6 | 5 | 13 | 25:46 | -21 | 23 |
| 15 |
|
FK Mladá Boleslav | 24 | 4 | 8 | 12 | 29:42 | -13 | 20 |
| 16 |
|
Zbrojovka Brno | 24 | 3 | 7 | 14 | 19:39 | -20 | 16 |
| 17 |
|
SFC Opava | 24 | 3 | 6 | 15 | 15:47 | -32 | 15 |
| 18 |
|
1. FK Příbram | 24 | 2 | 7 | 15 | 17:52 | -35 | 13 |
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| Relégation |
