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| 12.03.2021 | 18:00 | Slovan Liberec | - | FK Teplice | 2:1 (0:1) | |
| 13.03.2021 | 14:00 | SFC Opava | - | FK Pardubice | 1:0 (1:0) | |
| 16:00 | Baník Ostrava | - | 1. FK Příbram | 5:0 (2:0) | ||
| 16:00 | FC Fastav Zlín | - | Dynamo České Budějovice | 3:0 (1:0) | ||
| 14.03.2021 | 14:00 | Bohemians Praha 1905 | - | MFK Karviná | 2:0 (1:0) | |
| 16:00 | Zbrojovka Brno | - | FK Jablonec | 1:2 (0:1) | ||
| 18:30 | FK Mladá Boleslav | - | Slavia Praha | 0:3 (0:2) | ||
| 06.04.2021 | 16:30 | Sigma Olomouc | - | 1. FC Slovácko | 0:0 (0:0) | |
| 12.05.2021 | 18:00 | AC Sparta Praha | - | Viktoria Plzeň | 3:1 (0:1) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 23 | 19 | 4 | 0 | 66:15 | 51 | 61 |
| 2 |
|
AC Sparta Praha | 23 | 16 | 2 | 5 | 50:26 | 24 | 50 |
| 3 |
|
FK Jablonec | 23 | 14 | 3 | 6 | 39:26 | 13 | 45 |
| 4 |
|
Slovan Liberec | 23 | 12 | 6 | 5 | 36:20 | 16 | 42 |
| 5 |
|
1. FC Slovácko | 23 | 12 | 5 | 6 | 42:24 | 18 | 41 |
| 6 |
|
Viktoria Plzeň | 23 | 11 | 4 | 8 | 40:29 | 11 | 37 |
| 7 |
|
FK Pardubice | 23 | 10 | 4 | 9 | 24:30 | -6 | 34 |
| 8 |
|
Baník Ostrava | 23 | 9 | 6 | 8 | 32:23 | 9 | 33 |
| 9 |
|
Sigma Olomouc | 23 | 7 | 10 | 6 | 29:25 | 4 | 31 |
| 10 |
|
Dynamo České Budějovice | 23 | 7 | 9 | 7 | 26:31 | -5 | 30 |
| 11 |
|
FC Fastav Zlín | 23 | 8 | 5 | 10 | 24:29 | -5 | 29 |
| 12 |
|
Bohemians Praha 1905 | 23 | 7 | 7 | 9 | 25:27 | -2 | 28 |
| 13 |
|
MFK Karviná | 23 | 6 | 8 | 9 | 25:36 | -11 | 26 |
| 14 |
|
FK Teplice | 23 | 6 | 4 | 13 | 24:45 | -21 | 22 |
| 15 |
|
FK Mladá Boleslav | 23 | 4 | 7 | 12 | 27:40 | -13 | 19 |
| 16 |
|
Zbrojovka Brno | 23 | 3 | 6 | 14 | 18:38 | -20 | 15 |
| 17 |
|
SFC Opava | 23 | 3 | 6 | 14 | 15:43 | -28 | 15 |
| 18 |
|
1. FK Příbram | 23 | 2 | 6 | 15 | 16:51 | -35 | 12 |
| Champions League Quali. | |
| Europa League Quali. | |
| Relégation |
